Open AccessIn vitro cultivation of zygotic embryos from Murici (Byrsonima cydoniifolia A. Juss.): establishment, disinfection, and germination
Author(s) -
Cíntia de Oliveira Martendal,
Murilo Martins Bernardino,
Flávia Dionísio Pereira,
Fabiano Guimarães Silva,
Carlos César Evangelista de Menezes,
Alessandra Cristina Boffino de Almeida Monteiro Hara
Publication year - 2013
Publication title -
acta scientiarum. agronomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 28
eISSN - 1807-8621
pISSN - 1679-9275
DOI - 10.4025/actasciagron.v35i2.15402
Subject(s) - germination , seedling , sodium hypochlorite , horticulture , biology , botany , micropropagation , sterilization (economics) , agar , embryo culture , embryo , chemistry , tissue culture , in vitro , embryogenesis , bacteria , biochemistry , genetics , organic chemistry , economics , monetary economics , foreign exchange market , microbiology and biotechnology , foreign exchange
The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss.) using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5%) with or without 70% alcohol was used to avoid contamination because it was not toxic to murici embryos. Water-agar was the most appropriate culture medium for bud germination, and 50% WPM was appropriate for seedling growth