Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., Isolated from Soil

Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242 T and B156 T , were isolated from soil in South Korea. Cells of strain H242 T were oxidase-positive and non-motile short rods, while those of strain B156 T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C 16:0 , cyclo-C 17:0 , and summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242 T and B156 T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242 T and B156 T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242 T and B156 T was 24.6%. Strains H242 T and B156 T were most closely related to Ramlibacter ginsenosidimutans BXN5-27 T and Ramlibacter monticola G-3-2 T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242 T and R. ginsenosidimutans and between strain B156 T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242 T and B156 T represent two novel species of the genus Ramlibacter , for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242 T (=KACC 21667 T =JCM 33922 T ) and B156 T (=KACC 21665 T =JCM 33920 T ), respectively.