Mec1 Modulates Interhomolog Crossover and Interplays with Tel1 at Post Double-Strand Break Stages

During meiosis I, programmed DNA double-strand breaks (DSBs) occur to promote chromosome pairing and recombination between homologs. In Saccharomyces cerevisiae , Mec1 and Tel1, the orthologs of human ATR and ATM, respectively, regulate events upstream of the cell cycle checkpoint to initiate DNA repair. Tel1 ATM and Mec1 ATR are required for phosphorylating various meiotic proteins during recombination. This study aimed to investigate the role of Tel1 ATM and Mec1 ATR in meiotic prophase via physical analysis of recombination. Tel1 ATM cooperated with Mec1 ATR to mediate DSB-to-single end invasion transition, but negatively regulated DSB formation. Furthermore, Mec1 ATR was required for the formation of interhomolog joint molecules from early prophase, thus establishing a recombination partner choice. Moreover, Mec1 ATR specifically promoted crossover-fated DSB repair. Together, these results suggest that Tel1 ATM and Mec1 ATR function redundantly or independently in all post-DSB stages.