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Blue mold in citrus is caused by Penicillium italicum . In this study, the P. italicum -specific primers were developed for rapid detection based on the conserved genes RPB1 and RPB2 among Penicillium genomes. The two primer pairs RPB1-a and RPB1-b proved to be specific to detect P. italicum . The PCR assay among 39 fungal isolates and the colonial, pathogenic morphologies and molecular methods validated the specificity and reliability of these two primer pairs. This report provided a method and P. italicum -specific primers, which might greatly contribute to citrus postharvest industry.

Development of Penicillium italicum-Specific Primers for Rapid Detection among Fungal Isolates in Citrus