Development of Penicillium italicum-Specific Primers for Rapid Detection among Fungal Isolates in Citrus | Zendy

Kai Chen | Zendy; Zhonghuan Tian | Zendy; Fatang Jiang | Zendy; Chao-an Long | Zendy

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Development of Penicillium italicum-Specific Primers for Rapid Detection among Fungal Isolates in Citrus

Author(s) -

Kai Chen,

Zhonghuan Tian,

Fatang Jiang,

Chao-an Long

Publication year - 2019

Publication title -

journal of microbiology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 64

eISSN - 1738-8872

pISSN - 1017-7825

DOI - 10.4014/jmb.1904.04021

Subject(s) - penicillium italicum , biology , penicillium digitatum , primer (cosmetics) , blue mold , penicillium , microbiology and biotechnology , botany , citrus fruit , penicillium expansum , postharvest , chemistry , organic chemistry

Blue mold in citrus is caused by Penicillium italicum . In this study, the P. italicum -specific primers were developed for rapid detection based on the conserved genes RPB1 and RPB2 among Penicillium genomes. The two primer pairs RPB1-a and RPB1-b proved to be specific to detec P. italicum . The PCR assay among 39 fungal isolates and the colonial, pathogenic morphologies and molecular methods validated the specificity and reliability of these two primer pairs. This report provided a method and P. italicum -specific primers, which might greatly contribute to citrus postharvest industry.

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