Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase | Zendy

Xianghua Tang | Zendy; Tian-Bao Luo | Zendy; Xue Li | Zendy; Huanhuan Yang | Zendy; Yunjuan Yang | Zendy; Junjun Li | Zendy; Bo Xu | Zendy; Zunxi Huang | Zendy

Open Access

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Author(s) -

Xianghua Tang,

Tian-Bao Luo,

Xue Li,

Huanhuan Yang,

Yunjuan Yang,

Junjun Li,

Bo Xu,

Zunxi Huang

Publication year - 2018

Publication title -

journal of microbiology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 64

eISSN - 1738-8872

pISSN - 1017-7825

DOI - 10.4014/jmb.1805.05023

Subject(s) - rhizopus oryzae , mycelium , solid state fermentation , rhizopus , fermentation , food science , bran , water activity , chemistry , glycerol , botany , biology , water content , raw material , biochemistry , organic chemistry , geotechnical engineering , engineering

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, μ of mycelium reached to 0.15 h -1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research