Label-free rapid and simple detection of exonuclease III Activity with DNA Templated Copper Nanoclusters | Zendy

Chunghyun Lee | Zendy; Jongback Gang | Zendy

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Label-free rapid and simple detection of exonuclease III Activity with DNA Templated Copper Nanoclusters

Author(s) -

Chunghyun Lee,

Jongback Gang

Publication year - 2018

Publication title -

journal of microbiology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 64

eISSN - 1738-8872

pISSN - 1017-7825

DOI - 10.4014/jmb.1805.04060

Subject(s) - nanoclusters , dna , exonuclease , copper , chemistry , simple (philosophy) , combinatorial chemistry , biology , biochemistry , dna polymerase , organic chemistry , philosophy , epistemology

In this study, DNA-templated copper nanoclusters (DNA-CuNCs) were used to detect exonuclease III (Exo III) activity, which is important for the diagnosis and therapy of several diseases. The results of this study showed that Exo III was affected by the concentrations of magnesium ions and sodium ions, and its optimal conditions for cleavage were 5 mM Mg2+ and less than 25 mM Na+. With a blunt-end DNA, more than 98% of DNA was digested by Exo III. As expected, with two or four cytosines in the terminal position of a 4-base overhanging DNA such as 5'-GGCC-3' and 5'-CCCC-3', there was little cleavage by Exo III compared to with a blunt-end DNA.

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