Genetic and functional analysis of the DKxanthene biosynthetic gene cluster from Myxococcusstipitatus DSM 14675 | Zendy

Hyesook Hyun | Zendy; Sunjin Lee | Zendy; Jong Suk Lee | Zendy; Kyungyun Cho | Zendy

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Genetic and functional analysis of the DKxanthene biosynthetic gene cluster from Myxococcusstipitatus DSM 14675

Author(s) -

Hyesook Hyun,

Sunjin Lee,

Jong Suk Lee,

Kyungyun Cho

Publication year - 2018

Publication title -

journal of microbiology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 64

eISSN - 1738-8872

pISSN - 1017-7825

DOI - 10.4014/jmb.1802.02045

Subject(s) - myxococcus xanthus , myxobacteria , gene cluster , biology , gene , polyketide , biochemistry , polyketide synthase , sequence analysis , microbiology and biotechnology , biosynthesis , genetics , bacteria , mutant

DKxanthenes are a class of yellow secondary metabolites produced by myxobacterial genera Myxococcus and Stigmatella . We identified a putative 49.5 kb DKxanthene biosynthetic gene cluster from Myxococcus stipitatus DSM 14675 by genomic sequence and mutational analysis. The cluster was comprisedof 15 genes (MYSTI_06004-MYSTI_06018) encoding polyketide synthases, non-ribosomal peptide synthases, and proteins with unknown functions. Disruption of the genes by plasmid insertion resulted in defects in the production of yellow pigments. High-performance liquid chromatography and liquid chromatography-tandem mass spectrometry analysis indicated that the yellow pigments produced by M. stipitatus DSM 14675 might be noble DKxanthene derivatives. M. stipitatus did not require DKxanthenes for the formation of heat-resistant viable spores, unlike Myxococcus xanthus . Furthermore, DKxanthenes showed growth inhibitory activity against the fungi Aspergillus niger , Candida albicans , and Rhizopus stolonifer .

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