Differentiation in Nitrogen-Converting Activity and Microbial Community Structure between Granular Size Fractions in a Continuous Autotrophic Nitrogen Removal Reactor | Zendy

Feiyue Qian | Zendy; Xi Chen | Zendy; Jianfang Wang | Zendy; Yao-Liang Shen | Zendy; Jun-Jun Gao | Zendy; Juan Mei | Zendy

Open Access

Differentiation in Nitrogen-Converting Activity and Microbial Community Structure between Granular Size Fractions in a Continuous Autotrophic Nitrogen Removal Reactor

Author(s) -

Feiyue Qian,

Xi Chen,

Jianfang Wang,

Yao-Liang Shen,

Jun-Jun Gao,

Juan Mei

Publication year - 2017

Publication title -

journal of microbiology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 64

eISSN - 1738-8872

pISSN - 1017-7825

DOI - 10.4014/jmb.1705.05042

Subject(s) - nitrospira , chemistry , nitrite , anammox , autotroph , nitrogen , batch reactor , nitrosomonas , sequencing batch reactor , ammonium , anoxic waters , nitrification , bacteria , environmental chemistry , denitrification , biology , nitrate , environmental engineering , biochemistry , organic chemistry , denitrifying bacteria , wastewater , genetics , engineering , catalysis

The differentiations in nitrogen-converting activity and microbial community structure between granular size fractions in a continuous completely autotrophic nitrogen removal over nitrite (CANON) reactor, having a superior specific nitrogen removal rate of 0.24 g/(g VSS·d), were investigated by batch tests and high-throughput pyrosequencing analysis, respectively. Results revealed that a high dissolved oxygen concentration (>1.8 mg/l) could result in efficient nitrite accumulation with small granules (0.2-0.6 mm in diameter), because aerobic ammonium-oxidizing bacteria (genus Nitrosomonas ) predominated therein. Meanwhile, intermediate size granules (1.4-2.0 mm in diameter) showed the highest nitrogen removal activity of 40.4 mg/(g VSS·h) under sufficient oxygen supply, corresponding to the relative abundance ratio of aerobic to anaerobic ammonium-oxidizing bacteria (genus Candidatus Kuenenia) of 5.7. Additionally, a dual substrate competition for oxygen and nitrite would be considered as the main mechanism for repression of nitrite-oxidizing bacteria, and the few Nitrospira spp. did not remarkably affect the overall performance of the reactor. Because all the granular size fractions could accomplish the CANON process independently under oxygen limiting conditions, maintaining a diversity of granular size would facilitate the stability of the suspended growth CANON system.

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