Enzymatic Production of 15-Hydroxyeicosatetraenoic Acid from Arachidonic Acid by Using Soybean Lipoxygenase | Zendy

Baek-Joong Kim | Zendy; KyungChul Shin | Zendy; DeokKun Oh | Zendy

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Enzymatic Production of 15-Hydroxyeicosatetraenoic Acid from Arachidonic Acid by Using Soybean Lipoxygenase

Author(s) -

Baek-Joong Kim,

KyungChul Shin,

DeokKun Oh

Publication year - 2014

Publication title -

journal of microbiology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 64

eISSN - 1738-8872

pISSN - 1017-7825

DOI - 10.4014/jmb.1310.10054

Subject(s) - arachidonic acid , lipoxygenase , hydroxyeicosatetraenoic acid , enzyme , metabolite , biochemistry , chemistry , yield (engineering) , secondary metabolite , chromatography , materials science , metallurgy , gene

15-Hydroxyeicosatetraenoic acid (HETE), as a mammalian biologically active metabolite, has anticarcinogenic effect. The conditions of producing 15-HETE from arachidonic acid by using soybean lipoxygenase were optimal at pH 8.5 and 20°C with 9 g/l arachidonic acid, 54.4 U/ml soybean lipoxygenase, and 4% methanol. Under these optimized conditions, the enzyme produced 9.5 g/l 15-HETE after 25 min, with a molar conversion yield of 99% and a productivity of 22.8 g l(-1) h(-1). To the best of our knowledge, this is the first biotechnological production of 15-HETE.

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