Open AccessSevere Iron Deficiency Decreases Both Bone Formation and Bone Resorption in Rats
Author(s) -
Seishi Katsumata,
Rie Katsumata-Tsuboi,
Mariko Uehara,
Kazuharu Suzuki
Publication year - 2009
Publication title -
the journal of nutrition/the journal of nutrition
Language(s) - English
Resource type - Journals
eISSN - 1541-6100
pISSN - 0022-3166
DOI - 10.3945/jn.108.093757
Subject(s) - endocrinology , medicine , deoxypyridinoline , bone resorption , bone mineral , chemistry , osteocalcin , osteoclast , hemoglobin , osteoporosis , alkaline phosphatase , biochemistry , receptor , enzyme
The purpose of this study was to clarify the manner in which dietary iron deficiency decreased bone mineral density (BMD) in rats. Eighteen 3-wk-old male Wistar rats were divided into 3 groups of 6 rats each. The rats in 2 of the 3 groups had free access to a control diet (C group) or an iron-deficient diet (ID group) for 4 wk. The rats in the third group (PF group) were pair-fed the control diet to the mean intake of the ID group. Compared with the C and PF groups, hematocrit and hemoglobin concentrations were significantly reduced and bone mineral content and BMD of the femur were significantly lower in the ID group. Bone histomorphometric parameters showed that the bone formation rate and osteoclast surface in the lumbar vertebra were significantly reduced in the ID group compared with the C and PF groups. Furthermore, dietary iron deficiency decreased serum 1,25-dihydroxycholecalciferol, insulin-like growth factor-I, and osteocalcin concentrations and urinary excretion of deoxypyridinoline. These results suggest that severe iron deficiency decreases not only bone formation but also bone resorption.