Super-resolution Fluorescence Microscopy Based on Single-molecule Localization

Fluorescence microscopy is one of the most common techniques used in biology. Fluorescence microscopy has many advantages, but its poor resolution due to the diffraction limit of light is a weakness of this technique. As a result of efforts to overcome this limitation of fluorescence microscopy, several techniques were developed to break the diffraction limit of light. In this article, recent advances in super-resolution fluorescence microscopy based on single-molecule localization is introduced. The principle of the technique and several factors that can affect the resolution of the system are explained. Several methods to obtain better resolution and to optimize experimental conditions are developed, and new findings that were made by using this technique will be presented.