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The effects of genotype and altitude of the growing location on physical, chemical, and phytochemical properties of strawberry fruits were investigated. Eight strawberry genotypes obtained from diverse breeding programs were selected. The genotypes were grown at three altitudes: in Antakya (117 m), Urumu (443 m), and Saksak (755 m). The results indicated that genotype and growing location had a significant impact on both physico-chemical and phytochemical characteristics. Genotypes explained 36%–51% of total variance for fruit weight (FW), total soluble solids (TSS), total acidity (TA), color incidence chroma (C), hue ( h° ), citric acid, and total monomeric anthocyanin (TMA). Altitudes explained 23%–50% of total variance for color L, total phenolic content (TPC), glucose, fructose, and total sugar. The genotype effect was larger than that of the different altitude conditions for most of the physico-chemical and phytochemical component variables in the experiment, showing that breeding for fruit quality properties may be successful.