A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation | Zendy

Erkan Mozioğlu | Zendy; Müslüm Akgöz | Zendy; Candan Tamerler | Zendy; Zühtü Tanıl KOCAGÖZ | Zendy

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A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation

Author(s) -

Erkan Mozioğlu,

Müslüm Akgöz,

Candan Tamerler,

Zühtü Tanıl KOCAGÖZ

Publication year - 2013

Publication title -

turkish journal of biology

Language(s) - English

Resource type - Journals

eISSN - 1303-6092

pISSN - 1300-0152

DOI - 10.3906/biy-1308-7

Subject(s) - salmonella enterica , genomic dna , biology , dna , isolation (microbiology) , dna extraction , isothiocyanate , salmonella , escherichia coli , serotype , microbiology and biotechnology , bacteria , polymerase chain reaction , genetics , biochemistry , gene

A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.

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