Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells | Zendy

HsienKuo Chin | Zendy; ChiTing Horng | Zendy; YiShan Liu | Zendy; ChiCheng Lu | Zendy; ChenYing Su | Zendy; PeiSyuan Chen | Zendy; HongYi Chiu | Zendy; FuuJen Tsai | Zendy; Pochuen Shieh | Zendy; JaiSing Yang | Zendy

Open Access

Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells

Author(s) -

HsienKuo Chin,

ChiTing Horng,

YiShan Liu,

ChiCheng Lu,

ChenYing Su,

PeiSyuan Chen,

HongYi Chiu,

FuuJen Tsai,

Pochuen Shieh,

JaiSing Yang

Publication year - 2018

Publication title -

oncology reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.094

H-Index - 96

eISSN - 1791-2431

pISSN - 1021-335X

DOI - 10.3892/or.2018.6312

Subject(s) - kaempferol , protein kinase b , angiogenesis , pi3k/akt/mtor pathway , mapk/erk pathway , umbilical vein , cancer research , signal transduction , chemistry , biology , microbiology and biotechnology , quercetin , biochemistry , in vitro , antioxidant

Anti-angiogenesis is one of the most general clinical obstacles in cancer chemotherapy. Kaempferol is a flavonoid phytochemical found in many fruits and vegetables. Our previous study revealed that kaempferol triggered apoptosis in human umbilical vein endothelial cells (HUVECs) by ROS‑mediated p53/ATM/death receptor signaling. However, the anti‑angiogenic potential of kaempferol remains unclear and its underlying mechanism warranted further exploration in VEGF‑stimulated HUVECs. In the present study, kaempferol significantly reduced VEGF‑stimulated HUVEC viability. Kaempferol treatment also inhibited cell migration, invasion, and tube formation in VEGF‑stimulated HUVECs. VEGF receptor‑2 (VEGFR‑2), and its downstream signaling cascades (such as AKT, mTOR and MEK1/2‑ERK1/2) were reduced as determined by western blotting and kinase activity assay in VEGF‑stimulated HUVECs after treatment with kaempferol. The present study revealed that kaempferol may possess angiogenic inhibition through regulation of VEGF/VEGFR‑2 and its downstream signaling cascades (PI3K/AKT, MEK and ERK) in VEGF-stimulated endothelial cells.

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