Open AccessProteomic identification of target proteins following Drosha knockdown in cervical cancer
Author(s) -
Jun Zhou,
Jing Cai,
Zaiju Huang,
Hui Ding,
Junjie Wang,
Jinghui Jia,
Yong Zhao,
Da Huang,
Zehua Wang
Publication year - 2013
Publication title -
oncology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.094
H-Index - 96
eISSN - 1791-2431
pISSN - 1021-335X
DOI - 10.3892/or.2013.2672
Subject(s) - drosha , downregulation and upregulation , gene knockdown , microrna , biology , cancer research , blot , oncogene , microbiology and biotechnology , cancer , chemistry , cell cycle , apoptosis , rna interference , biochemistry , genetics , rna , gene
The nuclear microRNA (miRNA) processing enzyme Drosha is upregulated in cervical cancer, and its overexpression is related to an invasive tumour phenotype. However, the mechanisms that underlie this effect remain poorly understood. The aim of this study was to identify the potential targets of Drosha in cervical cancer. Here, we demonstrated that Drosha knockdown (Drosha-KD) inhibited proliferation, colony formation and the migration of cervical cancer cells in vitro. A global upregulation of proteins in Drosha-KD cells was revealed by two-dimensional gel electrophoresis (2-DE). Eighteen proteins were identified by liquid chromatography and tandem mass spectrometry technology (LC-MS/MS) from 21 selected protein spots that exhibited significant alterations in Drosha-KD cells. The majority of the identified proteins have been previously associated with tumour formation. The downregulation of tubulin 5β in Drosha-KD cervical cancer cells was further confirmed by western blotting. Our results suggest that Drosha affects the biological activity of cervical cancer cells by regulating the expression of numerous tumour-associated proteins.
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