Role of the ABCE1 gene in human lung adenocarcinoma

ATP-binding cassette transporter E1 (ABCE1), also known as RLI (RNase Linhibitor), is a new type of endoribonuclease inhibitor, which can specificallybind to RNase L and abolish its effect. ABCE1 binds to eIF2α and eIF5 to forma pre-translation initiation complex, suggesting its crucial role in cell growth,development and certain pathological processes. To probe the role of ABCE1 inthe development and progress of human lung adenocarcinoma, we first detected thechanges of its mRNA and protein expression in tissues, and found a high expressionlevel of ABCE1 in human lung adenocarcinoma tissues and metastatic lymph nodes,which was also correlated with clinical stages. Moreover, human lung adenocarcinomaA549 cells were infected with lentiviral vectors containing ABCE1-specific shRNA,and resulted in significant inhibition of cell growth. Using microarray assay,a number of differentially expressed genes were found after ABCE1 suppression.Our results demonstrated the potential role of ABCE1 in human lung adenocarcinoma,which may provide some molecular basis for the mechanisms of development and progressof human lung adenocarcinoma, and help to find new pharmacological targets.