Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB | Zendy

Qiuye Ma | Zendy; Yidong Yu | Zendy; Linlin Dai | Zendy; Xuehua Qu | Zendy; Shan Cong | Zendy; Hongsuo Liang | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB

Author(s) -

Qiuye Ma,

Yidong Yu,

Linlin Dai,

Xuehua Qu,

Shan Cong,

Hongsuo Liang

Publication year - 2017

Publication title -

oncology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.766

H-Index - 54

eISSN - 1792-1082

pISSN - 1792-1074

DOI - 10.3892/ol.2017.6373

Subject(s) - survivin , gene knockdown , fibrosarcoma , oncogene , cancer research , apoptosis , cell growth , caspase 3 , microbiology and biotechnology , protein kinase b , chemistry , biology , blot , cell cycle , signal transduction , programmed cell death , biochemistry , genetics , gene

The present study explored the effect of telomerase reverse transcriptase (TERT) on the growth and apoptosis of fibrosarcoma, and investigated the potential molecular signalling pathways underlying its effect. A plasmid was constructed in order to overexpress TERT and siRNA was used to knockdown TERT. The effect of TERT on fibrosarcoma cells in vitro was studied by performing reverse transcription-quantitative PCR and western blotting to determine the expression of p53, survivin, caspase-3, caspase-7 and PKB. Knockdown of TERT suppressed cell growth, decreased fibrosarcoma volume, decreased survivin and PKB expression, and increased caspase-3 expression. The results of the present study suggest that TERT regulates the growth of fibrosarcoma in vitro and in vivo , and that this is associated with the expression of caspase-3 and survivin, in addition to the PKB signalling pathway.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research