Overexpression and lack of copy number variation in the BMI-1 gene in human glioma | Zendy

M.K. Sibin | Zendy; Dhananjaya Ishwara Bhat | Zendy; Lavanya Choppavarapu | Zendy; Arati Suvatha | Zendy; Chetan Ghati Kasturirangan | Zendy

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Overexpression and lack of copy number variation in the BMI-1 gene in human glioma

Author(s) -

M.K. Sibin,

Dhananjaya Ishwara Bhat,

Lavanya Choppavarapu,

Arati Suvatha,

Chetan Ghati Kasturirangan

Publication year - 2015

Publication title -

oncology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.766

H-Index - 54

eISSN - 1792-1082

pISSN - 1792-1074

DOI - 10.3892/ol.2015.3686

Subject(s) - oncogene , molecular medicine , cell cycle , glioma , copy number variation , gene , biology , variation (astronomy) , genetics , human genetics , cancer , cancer research , genome , physics , astrophysics

Malignant gliomas are neoplasms of the brain that are associated with a poor prognosis. The B-cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) gene is one of the major cancer stem cell factors responsible for treatment failure in glioma. In the present study, the DNA-RNA-protein alterations in the BMI-1 gene were assessed in 50 glioma samples. Copy number variations in the BMI-1 gene were analyzed using SYBR® Green quantitative polymerase chain reaction. Gene expression analysis was performed using a Taqman assay and protein quantitation was performed using western blotting. A comparative Ct analysis showed the absence of copy number variations in all glioma samples. BMI-1 mRNA expression was found to be overexpressed in 36 out of 50 samples (72.0%), and 37 out of 50 samples showed overexpression (74.0%) of BMI-1 protein; this was statistically significant when compared with non-glioma tissues. It was observed that the protein and RNA expression in glioma were concordant. In this study on the BMI-1 gene, transcription and translation in glioma were observed and BMI-1 overexpression was found to be a common phenomenon.

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