Inflammatory mediators, tumor necrosis factor-α and interferon-γ, induce EMT in human PTC cell lines | Zendy

Nannan Lv | Zendy; Yun Gao | Zendy; Haixia Guan | Zendy; Dan Wu | Zendy; Shuangning Ding | Zendy; Weiping Teng | Zendy; Zhongyan Shan | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Inflammatory mediators, tumor necrosis factor-α and interferon-γ, induce EMT in human PTC cell lines

Author(s) -

Nannan Lv,

Yun Gao,

Haixia Guan,

Dan Wu,

Shuangning Ding,

Weiping Teng,

Zhongyan Shan

Publication year - 2015

Publication title -

oncology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.766

H-Index - 54

eISSN - 1792-1082

pISSN - 1792-1074

DOI - 10.3892/ol.2015.3518

Subject(s) - vimentin , tumor necrosis factor alpha , oncogene , cancer research , epithelial–mesenchymal transition , cell migration , interferon , biology , cell cycle , gentamicin protection assay , cell , downregulation and upregulation , molecular medicine , immunology , cancer , metastasis , immunohistochemistry , gene , biochemistry , genetics

Inflammatory mediators, tumor necrosis factor (TNF)-α and interferon (IFN)-γ, promote adverse outcomes in numerous types of cancer; however, their role in papillary thyroid cancer (PTC) remains unclear. The aim of the present study was to investigate the influence of TNF-α and IFN-γ on the migration, invasion and epithelial-mesenchymal transition (EMT) of the three PTC cell lines, TPC-1, BCPAP and K1. The effect of TNF-α and IFN-γ on cell migration and invasion was assessed by wound-healing and Transwell assays. In addition, the mRNA and protein expression levels of the EMT makers, E-cadherin, N-cadherin and vimentin, were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunoblot analysis. The wound-healing and Transwell experiments revealed that TNF-α and IFN-γ increased the migratory and invasive behavior of PTC cells (P<0.05). RT-qPCR revealed that TNF-α and IFN-γ downregulated E-cadherin mRNA, while they upregulated N-cadherin and vimentin mRNA expression levels. These results were further confirmed by the immunoblot analysis. The results of the present study suggest that TNF-α and IFN-γ induce EMT and malignant progression in human PTC cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research