Tumor suppressor microRNA-34a inhibits cell proliferation by targeting Notch1 in renal cell carcinoma | Zendy

Changcun Zhang | Zendy; Ren Mo | Zendy; BINDE YIN | Zendy; Libin Zhou | Zendy; Yongchao Liu | Zendy; Jie Fan | Zendy

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Tumor suppressor microRNA-34a inhibits cell proliferation by targeting Notch1 in renal cell carcinoma

Author(s) -

Changcun Zhang,

Ren Mo,

BINDE YIN,

Libin Zhou,

Yongchao Liu,

Jie Fan

Publication year - 2014

Publication title -

oncology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.766

H-Index - 54

eISSN - 1792-1082

pISSN - 1792-1074

DOI - 10.3892/ol.2014.1931

Subject(s) - oncogene , cell cycle , molecular medicine , suppressor , renal cell carcinoma , microrna , cancer research , cell , cell growth , a431 cells , apoptosis , cyclin dependent kinase 8 , clear cell renal cell carcinoma , cancer , biology , medicine , oncology , microbiology and biotechnology , signal transduction , gene , notch signaling pathway , genetics

MicroRNA-34a (miR-34a) is a tumor suppressive microRNA, which induces G1 arrest, apoptosis and senescence by repressing the expression of multiple oncogenes. This study aimed to investigate the biological function and molecular mechanisms of miR-34a in human renal cell carcinoma (RCC) cells. Quantitative polymerase chain reaction (qPCR) revealed that miR-34a expression was significantly downregulated in eight of the 10 (80%) RCC tissues compared with adjacent normal tissues. In RCC cell lines, several other target genes of miR-34a were dysregulated at the mRNA level when the expression of miR-34a was elevated. In addition, western blot analysis and qPCR revealed that forced expression of miR-34a downregulated the expression of Notch1 at the protein and mRNA level. The Cell Counting Kit-8 identified that transient forced expression of miR-34a inhibited cell growth and resulted in cell cycle arrest, which was evaluated by flow cytometry. Our data demonstrated that miR-34a inhibits cell proliferation by downregulating Notch1 in RCC cell lines.

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