Connexin 43-dependent tumor-suppressing effect of the Bowman-Birk protease inhibitor on M5076 ovarian sarcoma-bearing mice | Zendy

Sakurai | Zendy

Open Access

Connexin 43-dependent tumor-suppressing effect of the Bowman-Birk protease inhibitor on M5076 ovarian sarcoma-bearing mice

Author(s) -

Sakurai

Publication year - 2008

Publication title -

molecular medicine reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.727

H-Index - 56

eISSN - 1791-3004

pISSN - 1791-2997

DOI - 10.3892/mmr_00000014

Subject(s) - connexin , sarcoma , oncogene , ovarian cancer , endocrinology , apoptosis , biology , gap junction , ovarian tumor , medicine , molecular medicine , cell cycle , cancer , cancer research , intracellular , pathology , microbiology and biotechnology , biochemistry

The present study was designed to confirm whether the Bowman-Birk inhibitor (BBI) induces an increase in p27 accumulation without S phase kinase-associated protein 2 (skp2) degradation by means of the expression of connexin (Cx) 43 as a gap junctional intercellular communication (GJIC)-dependent pathway in mice with M5076 ovarian sarcoma. M5076 ovarian sarcomas (1x105 cells/animal) were subcutaneously transplanted onto the backs of BDF1 mice receiving 10, 20 or 40 mg/kg of purified BBI intraperitoneally. Relative tumor weight (p<0.01, r=0.503) was negatively correlated with the dose of BBI. In contrast, the relative density of Cx43 mRNA (p<0.01, r=0.570) and Cx43 (p<0.01, r=0.718) was positively correlated with the dose of BBI, as were p21 (p<0.01, r=0.633), p27 (p<0.01, r=0.561) and skp2 (p<0.01, r=0.733). We therefore suggest that the anti-carcinogenic effects of BBI induce negative growth control by means of an increase in p27 accumulation caused by the expression of Cx43 as a GJIC pathway.

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