Open AccessmiR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2
Author(s) -
Mingjun Lu,
Lingling Xu,
Min Wang,
Tao Guo,
Fuquan Luo,
Nan Su,
Shanghui Yi,
Tao Chen
Publication year - 2018
Publication title -
molecular medicine reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.727
H-Index - 56
eISSN - 1791-3004
pISSN - 1791-2997
DOI - 10.3892/mmr.2018.8903
Subject(s) - wnt signaling pathway , mesenchymal stem cell , biology , microrna , oncogene , ectopic expression , microbiology and biotechnology , bone marrow , stem cell , cell cycle , cellular differentiation , cancer research , signal transduction , in vitro , apoptosis , cell culture , immunology , gene , genetics
To investigate the role of microRNA (miR)‑149 in the cardiac differentiation of mouse bone marrow mesenchymal stem cells (MSCs) in vitro, MSCs were infected with a lentivirus overexpressing miR‑149 and the effect on cardiac differentiation was determined. The quantitative polymerase chain reaction results demonstrated that miR‑149 promoted the expression of cardiac‑specific markers in MSCs. Western blotting and a luciferase activity assay demonstrated that disabled homolog 2 (Dab2) was a direct target of miR‑149. Dab2 ectopic expression and Wnt/β‑catenin signaling pathway inhibition was able to reverse the increased expression of cardiac‑specific markers induced by miR‑149. In conclusion, miR‑149 was able to target Dab2 and promote the cardiac differentiation of mouse MSCs in vitro, which depended upon the Wnt/β‑catenin signaling pathway.
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