Downregulation of Id1 by small interfering RNA in gastric cancer inhibits cell growth via the Akt pathway | Zendy

Guang Yang | Zendy; Yan Zhang | Zendy; Jianjun Xiong | Zendy; Jing Wu | Zendy; Changfu Yang | Zendy; Hongbing Huang | Zendy; Zhenyu Zhu | Zendy

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Downregulation of Id1 by small interfering RNA in gastric cancer inhibits cell growth via the Akt pathway

Author(s) -

Guang Yang,

Yan Zhang,

Jianjun Xiong,

Jing Wu,

Changfu Yang,

Hongbing Huang,

Zhenyu Zhu

Publication year - 2012

Publication title -

molecular medicine reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.727

H-Index - 56

eISSN - 1791-3004

pISSN - 1791-2997

DOI - 10.3892/mmr.2012.749

Subject(s) - downregulation and upregulation , cell cycle , oncogene , cell growth , cancer research , biology , cancer cell , cancer , cell , protein kinase b , small interfering rna , pi3k/akt/mtor pathway , a431 cells , microbiology and biotechnology , cell culture , transfection , signal transduction , gene , biochemistry , genetics

Inhibitor of differentiation or DNA binding (Id1) is a member of the helix-loop-helixtranscription factor family that is overexpressed in various types of cancer,including gastric carcinoma. Previous studies showed that Id1 is a prognosticmarker in patients with gastric cancer. However, the role of Id1 in the proliferationof human gastric cancer cells has yet to be clarified. In the present study, wedownregulated the Id1 gene in SGC-7901 gastric cancer cells by RNA interference,and we also constructed a recombinant plasmid-expressing Id1 to investigate itseffects on the proliferation of SGC-7901 cells. Results showed that the downregulationof Id1 inhibited proliferation of SGC-7901 cells, while the upregulation of Id1had no effect on SGC-7901 cell proliferation. The potential mechanism was alsoinvestigated. The changes of certain proteins associated with cell proliferation,apoptosis and the cell cycle were detected by western blotting. Furthermore, wedemonstrated a positive correlation between Id1 and phospho-Akt expression inSGC-7901 cells.

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