Isolation, culture and biological characteristics of multipotent porcine tendon-derived stem cells | Zendy

Jinjuan Yang | Zendy; Qianjun Zhao | Zendy; Kunfu Wang | Zendy; Caiyun Ma | Zendy; Hao Liu | Zendy; Yingjie Liu | Zendy; Weijun Guan | Zendy

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Isolation, culture and biological characteristics of multipotent porcine tendon-derived stem cells

Author(s) -

Jinjuan Yang,

Qianjun Zhao,

Kunfu Wang,

Caiyun Ma,

Hao Liu,

Yingjie Liu,

Weijun Guan

Publication year - 2018

Publication title -

international journal of molecular medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.048

H-Index - 90

eISSN - 1791-244X

pISSN - 1107-3756

DOI - 10.3892/ijmm.2018.3545

Subject(s) - cd90 , stem cell , multipotent stem cell , biology , wound healing , mesenchymal stem cell , pathology , microbiology and biotechnology , cd34 , immunology , medicine , progenitor cell

Tendon-derived stem cells (TDSCs), a postulated multi-potential stem cell population, play significant role in the postnatal replenishment of tendon injuries. However, the majority of experimental materials were obtained from horse, rat, human and rabbit, but rarely from pig. In this research, 1‑day‑old pig was chosen as experimental sample source to isolate and culture TDSCs in vitro. Specific markers of TDSCs were then characterized by immunofluorescence and reverse transcription polymerase chain reaction (RT‑PCR) assays. The results showed that TDSCs could be expanded for 11 passages in vitro. The expression of specific markers, such as collagen Ⅰ, collagen Ⅲ, α‑smooth muscle actin (α‑SMA), CD105 and CD90 were observed by immunofluorescence and RT‑PCR. TDSCs were induced to differentiate into adipocytes, osteoblasts and chondrocytes, respectively. These results suggest that TDSCs isolated from porcine tendon exhibit the characteristics of multipotent stem cells. TDSCs, therefore, may be potential candidates for cellular transplantation therapy and tissue engineering in tendon injuries.

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