Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma | Zendy

Wing Chun Tang | Zendy; Chung Chui | Zendy; Sarwat Fatima | Zendy; Stanton Kok | Zendy; K.C. Pak | Zendy; Tian Ou | Zendy; Kin Hui | Zendy; Mei Mei Wong | Zendy; John Wong | Zendy; Simon Law | Zendy; Sai Wah Tsao | Zendy; Alfred K. Lam | Zendy; Philip Beh | Zendy; Gopesh Srivastava | Zendy; Kwok W. Ho | Zendy; A.S.C. Chan | Zendy; Johnny Tang | Zendy

Open Access

Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

Author(s) -

Wing Chun Tang,

Chung Chui,

Sarwat Fatima,

Stanton Kok,

K.C. Pak,

Tian Ou,

Kin Hui,

Mei Mei Wong,

John Wong,

Simon Law,

Sai Wah Tsao,

Alfred K. Lam,

Philip Beh,

Gopesh Srivastava,

Kwok W. Ho,

A.S.C. Chan,

Johnny Tang

Publication year - 2007

Publication title -

international journal of molecular medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.048

H-Index - 90

eISSN - 1791-244X

pISSN - 1107-3756

DOI - 10.3892/ijmm.19.6.953

Subject(s) - telomerase , oncogene , cell cycle , cell , molecular medicine , esophageal squamous cell carcinoma , cancer research , biology , basal cell , apoptosis , carcinoma , medicine , pathology , gene , genetics

Previous studies have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) exhibited apoptotic properties in various solid and non-solid tumors in vitro. However, the inhibitory actions of GSE on oncogenic expression and telomerase activity in esophageal squamous cell carcinoma (ESCC) have not been studied before. In the present study, the anti-cancer effects of GSE were demonstrated in three ESCC cell lines (HKESC-1, HKESC-2 and SLMT-1) by MTS and anchorage-independent clongen-icity assays, expression studies on oncogenes at 11q13 (CCND1, INT2, FGF4 and EMS1) and real-time quantitative telomeric repeat amplification protocol assay to show the inhibitory effect of GSE on telomerase in ESCC. The means of MTS50 of GSE for the ESCC cell lines and non-tumor NIH 3T3 cells were 21 and 163 µg/ml respectively. The anchorage-independent clongenicity assay showed that SLMT-1 cells lost their colony-forming potential which was dose-dependent to GSE. Moreover, GSE demonstrated dose-dependent suppression on the expression of INT2, EMS1 and FGF4, and inhibition of telomerase activity in the ESCC cell lines. Our overall results thus provide the first evidence that the anti-cancer effects of GSE on ESCC involve the suppression of oncogenic expression and inhibition of telomerase activity. Our findings also offer a new opportunity for the future development of GSE as a novel anti-cancer agent for ESCC and possibly for other cancers.Department of Applied Biology and Chemical Technolog

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research