Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells | Zendy

Andrada Iftode | Zendy; George Drăghici | Zendy; Ioana Macașoi | Zendy; Iasmina Marcovici | Zendy; Dorina Coricovac | Zendy; Răzvan Drăgoi | Zendy; Alina Andreea Tischer | Zendy; Leda Kovatsi | Zendy; Aristidis Tsatsakis | Zendy; Octavian Creţu | Zendy; Cristina Dehelean | Zendy

Open Access

Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells

Author(s) -

Andrada Iftode,

George Drăghici,

Ioana Macașoi,

Iasmina Marcovici,

Dorina Coricovac,

Răzvan Drăgoi,

Alina Andreea Tischer,

Leda Kovatsi,

Aristidis Tsatsakis,

Octavian Creţu,

Cristina Dehelean

Publication year - 2020

Publication title -

experimental and therapeutic medicine

Language(s) - English

Resource type - Journals

eISSN - 1792-1015

pISSN - 1792-0981

DOI - 10.3892/etm.2020.9532

Subject(s) - dna methylation , epigenetics , cytotoxic t cell , methyltransferase , dnmt3b , carcinogenesis , methylation , microbiology and biotechnology , chemistry , dna methyltransferase , oncogene , cadmium , dnmt1 , biology , carcinogen , cancer research , cell cycle , cell , dna , in vitro , gene expression , biochemistry , gene , organic chemistry

Recent scientific evidence suggests a link between epigenetic changes (DNA methylation) and tumorigenesis. Moreover, a potential carcinogenic mechanism of cadmium was associated with changes in DNA methylation. In this study we investigated the impact of CdCl2 and CuSO4 aqueous solutions on DNA methylation in HT-29 cells by quantifying DNA methyltransferase (DNMT1, DNMT3A and DNMT3B) mRNA expression. Furthermore, we also studied the cytotoxic and anti-migratory potential of these substances. The results showed a dose-dependent decrease of viable cell percentage following 24 h of exposure (at concentrations of 0.05; 0.2; 1; 10 and 100 µg/ml), and an inhibitory effect on HT-29 cell migration capacity. In addition, RT-qPCR results showed that cadmium acts as a hypomethylating agent by suppressing DNMT expression, whereas copper acts as a hypermethylating compound by increasing DNMT expression. These findings suggest a cytotoxic potential of both cadmium and copper on HT-29 cells and their capacity to induce epigenetic changes.

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