Neural stem cell transplantation combined with erythropoietin for the treatment of spinal cord injury in rats | Zendy

Yan Zhao | Zendy; Zuo Yuan | Zendy; Jianming Jiang | Zendy; Huibo Yan | Zendy; Xiliang Wang | Zendy; Hunjun Huo | Zendy; Yu-long Xiao | Zendy

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Neural stem cell transplantation combined with erythropoietin for the treatment of spinal cord injury in rats

Author(s) -

Yan Zhao,

Zuo Yuan,

Jianming Jiang,

Huibo Yan,

Xiliang Wang,

Hunjun Huo,

Yu-long Xiao

Publication year - 2016

Publication title -

experimental and therapeutic medicine

Language(s) - English

Resource type - Journals

eISSN - 1792-1015

pISSN - 1792-0981

DOI - 10.3892/etm.2016.3677

Subject(s) - erythropoietin , neural stem cell , transplantation , spinal cord , medicine , spinal cord injury , immunohistochemistry , neuroprotection , glial fibrillary acidic protein , hindlimb , pathology , neurofilament , stem cell , andrology , biology , genetics , psychiatry

Spinal cord injury (SCI) comprises nerve and motor function disorders that may be caused by a variety of damaging factors and is challenging to treat. The aim of the present study was to investigate the regenerative effects of neural stem cell (NSC) transplantation combined with intraperitoneal injection of erythropoietin (EPO) on cross-sectional SCI in rats. A model of SCI was induced in 40 adult Wistar rats via the complete transection of the 10th thoracic vertebra (T10). The rats were allocated at random into 4 groups: Control, NSC, EPO and NSC + EPO groups (n=10 per group). Morphological alterations associated with axonal regeneration were detected using neurofilament (NF)-200 immunohistochemistry and immunofluorescence staining after 8 weeks. Basso, Beattie and Bresnahan (BBB) scoring was used to evaluate the recovery of hindlimb function. A total of 5 rats died following surgery, including 2 control rats and 1 rat each in the EPO, NSC and NSC + EPO groups. NSCs labeled with bromodeoxyuridine were observed to have survived and migrated in the spinal cord tissue after 8 weeks. Significant histomorphological differences were observed in the NSC and NSC + EPO groups compared with the EPO and control groups. Furthermore, the rats of the NSC + EPO group exhibited significantly enhanced axonal regeneration in the SCI area compared with the NSC group rats. The rats of the NSC and NSC + EPO groups exhibited significantly improved BBB scores compared with the EPO and control group rats at 7 days after treatment (P<0.05). In addition, the BBB scores of the NSC + EPO group were significantly improved compared with those of the three other groups at 7 days after surgery (P<0.05). Therefore, the results of the present study suggest that NSC transplantation combined with intraperitoneal injection of EPO may benefit the survival and regeneration of injured axons, and accelerate the repair of injured spinal cord tissue, thus facilitating the functional recovery of hindlimb locomotor function in rats.

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