Hedyotis diffusa Willd extract inhibits HT-29 cell proliferation via cell cycle arrest | Zendy

Minghe Lin | Zendy; Jiumao Lin | Zendy; Lihui Wei | Zendy; Wei Xu | Zendy; Zhenfeng Hong | Zendy; Qiaoyan Cai | Zendy; Jun Peng | Zendy; Zhu De-zeng | Zendy

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Hedyotis diffusa Willd extract inhibits HT-29 cell proliferation via cell cycle arrest

Author(s) -

Minghe Lin,

Jiumao Lin,

Lihui Wei,

Wei Xu,

Zhenfeng Hong,

Qiaoyan Cai,

Jun Peng,

Zhu De-zeng

Publication year - 2012

Publication title -

experimental and therapeutic medicine

Language(s) - English

Resource type - Journals

eISSN - 1792-1015

pISSN - 1792-0981

DOI - 10.3892/etm.2012.599

Subject(s) - cell cycle , cell growth , apoptosis , cyclin d1 , angiogenesis , cancer research , proliferating cell nuclear antigen , oncogene , cell cycle checkpoint , cancer , cell , molecular medicine , colorectal cancer , biology , chemistry , traditional medicine , pharmacology , medicine , biochemistry

Hedyotis diffusa Willd (HDW) has long been used as an important component in several Chinese medicine formulae to clinically treat various types of cancer, including colorectal cancer (CRC). Previously, we reported that HDW inhibits CRC growth via the induction of cancer cell apoptosis and the inhibition of tumor angiogenesis. In the present study, to further elucidate the mechanism of HDW-mediated antitumor activity, we investigated the effect of HDW ethanol extract (EEHDW) on the proliferation of HT-29 human colon carcinoma cells. We found that EEHDW reduced HT-29 cell viability and survival in a dose- and time-dependent manner. We also observed that EEHDW treatment blocked the cell cycle, preventing G1 to S progression, and reduced mRNA expression of pro-proliferative PCNA, Cyclin D1 and CDK4, but increased that of anti-proliferative p21. Our findings suggest that Hedyotis diffusa Willd may be an effective treatment for CRC via the suppression of cancer cell proliferation.

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