Effects of high-fat diet on 1,2-dimethylhydrazine-induced aberrant crypt foci and colorectal tumours in rats | Zendy

Guangying Qi | Zendy; Bufu Tang | Zendy; Lihua Zhou | Zendy; Hiroshi Jikihara | Zendy; Atsumi Kiwata | Zendy; Yuki Sakamoto | Zendy; Fang Tang | Zendy; Shengjun Xiao | Zendy; Zhenran Wang | Zendy; Qiuhui Wu | Zendy; Huiling Lü | Zendy; Zhenhua Wu | Zendy; Sien Zeng | Zendy; Fumio Shimamoto | Zendy

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Effects of high-fat diet on 1,2-dimethylhydrazine-induced aberrant crypt foci and colorectal tumours in rats

Author(s) -

Guangying Qi,

Bufu Tang,

Lihua Zhou,

Hiroshi Jikihara,

Atsumi Kiwata,

Yuki Sakamoto,

Fang Tang,

Shengjun Xiao,

Zhenran Wang,

Qiuhui Wu,

Huiling Lü,

Zhenhua Wu,

Sien Zeng,

Fumio Shimamoto

Publication year - 2015

Publication title -

biomedical reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.607

H-Index - 25

eISSN - 2049-9442

pISSN - 2049-9434

DOI - 10.3892/br.2015.433

Subject(s) - aberrant crypt foci , 1,2 dimethylhydrazine , basal (medicine) , colorectal cancer , medicine , dimethylhydrazine , endocrinology , obesity , crypt , adenocarcinoma , adenoma , physiology , cancer , biology , colonic disease , insulin

Obesity is one of the leading causes of numerous types of cancer. The present study investigated the impact of a high-fat diet on 1,2-dimethylhydrazine (DMH)-induced colorectal cancer (CRC) in F344 rats. A total of 16 male F344 rats aged 4 weeks were randomly divided into two groups (8 rats/group). Rats in group A were fed a basal diet with a moderate fat (MF) content, while rats in group B were fed a high-fat diet. Upon reaching 5 weeks of age, the rats were injected subcutaneously with DMH (20 mg/kg body weight). DMH was administered once a week for 8 consecutive weeks. All the rats were sacrificed 34 weeks after the first DMH injection and dissected to obtain samples of colorectal tissues. The tissues were examined under a microscope for the presence of aberrant crypt foci (ACFs) and subjected to histopathological analysis. The results showed that at the end of the 34-week experiment, body weights and visceral fat levels were significantly higher in the high-fat diet group compared to the basal diet group. In addition, the incidences of colorectal ACF, adenoma and adenocarcinoma were markedly elevated in the high-fat diet group compared to the basal diet group. These results indicate that the consumption of a high-fat diet promotes the development and progression of CRC and the control of fat intake may prevent CRC.

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