In vitro effects of emodin on peritoneal macrophage intercellular adhesion molecule-3 in a rat model of severe acute pancreatitis/systemic inflammatory response syndrome | Zendy

Qingqiang Ni | Zendy; Caiqing Zhang | Zendy; Kang Sun | Zendy; Chao Yin | Zendy; JIZHONG AN | Zendy; Dong Shang | Zendy

Open Access

In vitro effects of emodin on peritoneal macrophage intercellular adhesion molecule-3 in a rat model of severe acute pancreatitis/systemic inflammatory response syndrome

Author(s) -

Qingqiang Ni,

Caiqing Zhang,

Kang Sun,

Chao Yin,

JIZHONG AN,

Dong Shang

Publication year - 2013

Publication title -

biomedical reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.607

H-Index - 25

eISSN - 2049-9442

pISSN - 2049-9434

DOI - 10.3892/br.2013.178

Subject(s) - systemic inflammatory response syndrome , acute pancreatitis , molecular medicine , oncogene , in vitro , macrophage , intracellular , intercellular adhesion molecule 1 , inflammation , cell adhesion molecule , inflammatory response , cancer research , medicine , emodin , pancreatitis , pancreatic cancer , immunology , chemistry , biology , cancer , microbiology and biotechnology , cell cycle , sepsis , biochemistry

Rhubarb is often used in Chinese herbal medicine for the treatment of systemic inflammatory response syndrome (SIRS). Emodin is the main active constituent of rhubarb. This study was performed to investigate the in vitro effects of emodin and dexamethasone on peritoneal macrophage (pMΦ) phagocytosis and the expression of intercellular adhesion molecule-3 (ICAM-3). A total of 40 Sprague-Dawley (SD) rats were randomly divided into sham surgery (n=10) and model groups (n=30). After 24 h, pMΦs were harvested and the model group was randomly divided into three subgroups (n=10 rats/subgroup): the 5 μg/ml emodin, 0.1 μmol/ml dexamethasone and control groups. The drugs were administered following macrophage (MΦ) adhesion for 24 h. pMΦ phagocytosis was significantly increased in the emodin group compared to that in the control group. Moreover, pMΦ phagocytosis was significantly increased in the emodin group compared to that in the dexamethasone group. The expression of ICAM-3 was significantly increased in the emodin group compared to that in the control group. The expression of ICAM-3 was significantly increased in the emodin group compared to that in the dexamethasone group. The expression of ICAM-3 was significantly increased in the emodin and dexamethasone groups compared to that in the control group. pMΦ phagocytosis and ICAM-3 expression were significantly increased following emodin treatment compared to those in the control and dexamethasone groups, indicating that emodin may enhance pMΦ phagocytosis and apoptotic cell clearance by altering ICAM-3 expression.

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