Skin Tissue Surface Morphology and Quality of RNA and Protein Extracted from Fresh and Stabilized Human Cleft Lip and Palate Tissue | Zendy

Nurul Syazana Mohamad Shah | Zendy; Wan Azman Wan Sulaiman | Zendy; Ahmad Sukari Halim | Zendy; Sarina Sulong | Zendy

Open Access

Skin Tissue Surface Morphology and Quality of RNA and Protein Extracted from Fresh and Stabilized Human Cleft Lip and Palate Tissue

Author(s) -

Nurul Syazana Mohamad Shah,

Wan Azman Wan Sulaiman,

Ahmad Sukari Halim,

Sarina Sulong

Publication year - 2014

Publication title -

open access macedonian journal of medical sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.288

H-Index - 17

ISSN - 1857-9655

DOI - 10.3889/oamjms.2014.004

Subject(s) - nucleic acid , medicine , rna , morphology (biology) , homogenization (climate) , biopsy , pathology , scanning electron microscope , hard tissue , anatomy , dentistry , biochemistry , biology , gene , genetics , materials science , biodiversity , ecology , composite material

Cleft lip palate is a human congenital disorder worldwide and the study of this genetic disease requires molecular genetic analysis. This analysis required the use of nucleic acid and protein, thus good quality and quantity of its extraction is important. We are comparing RNA and protein extractions from tissue biopsy of cleft lip palate in both fresh and stabilized condition. Tissue morphology was also captured using Scanning Electron Microscopy (SEM) for any morphology differences. Tissue homogenization may destroy nucleic acid stability but not its morphology. Low RNA concentration from stabilized tissue was found. However, there was no crucial issue of protein extraction, degradation or concentration. Tissue morphology was slight different between normal and CL/P tissue

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