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Comparison of P1 and 16S rRNA genes for detection of Mycoplasma pneumoniae by nested PCR in adults in Zhejiang, China
Author(s) -
Zibo Zhou,
Xiangzhi Li,
Xiaojian Chen,
Lili Yao,
Pan ChangWang,
Huicong Huang,
Fangjun Luo,
Xiaoping Zheng,
Xiaojing Sun,
Feng Tan
Publication year - 2015
Publication title -
the journal of infection in developing countries
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 49
eISSN - 2036-6590
pISSN - 1972-2680
DOI - 10.3855/jidc.5149
Subject(s) - 16s ribosomal rna , mycoplasma pneumoniae , nested polymerase chain reaction , bacterial adhesin , biology , microbiology and biotechnology , gene , polymerase chain reaction , ribosomal rna , mycoplasma genitalium , virology , pneumonia , virulence , genetics , medicine , chlamydia trachomatis
Mycoplasma pneumoniae (M. pneumoniae) is the most common atypical pathogen that causes respiratory infections in humans. Laboratory tests are important in the diagnosis of M. pneumoniae because of the atypical features in clinical signs and symptoms. Nowadays, both the P1 adhesin gene and 16S ribosomal (r) RNA (rRNA) gene of M. pneumoniae have been widely detected by polymerase chain reaction (PCR). The purpose of the present study was to evaluate the most suitable target in the detection of M. pneumonia via nested PCR.

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