Comparison of bluetongue virus detection and quantitation methods in south India | Zendy

Subhra Subhadra | Zendy; Subrat Kumar | Zendy; Veluvarthy V.S. Suryanarayana | Zendy; D. Sreenivasulu | Zendy

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Comparison of bluetongue virus detection and quantitation methods in south India

Author(s) -

Subhra Subhadra,

Subrat Kumar,

Veluvarthy V.S. Suryanarayana,

D. Sreenivasulu

Publication year - 2014

Publication title -

the journal of infection in developing countries

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.322

H-Index - 49

eISSN - 2036-6590

pISSN - 1972-2680

DOI - 10.3855/jidc.4681

Subject(s) - real time polymerase chain reaction , virology , biology , melting curve analysis , virus , sybr green i , polymerase chain reaction , serotype , population , microbiology and biotechnology , taqman , gene , medicine , genetics , environmental health

Bluetongue (BT), a vector-borne viral disease, primarily affects sheep. Of the 26 serotypes of BTV identified so far, 22 are reported to be circulating in India. Due to an increase in vector population and delays in disease diagnosis, the BT control program heavily relies on rapid and confirmatory diagnosis. Polymerase chain reaction (PCR)-based real-time detection assays may be an ideal method to detect the BTV genome in animal blood at an early stage of infection.

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