Open AccessFirst Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.
Author(s) -
Sameh AbdelGhani,
Ellen Smith Moland,
Jennifer Ann Black,
Nancy D. Hanson,
Richard V. Goering,
Magdy A. Amine,
Amal E. Saafan,
Maha Gaafar,
M. Y. A. Younan,
Kenneth S. Thomson
Publication year - 2009
Publication title -
the journal of infection in developing countries
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 49
eISSN - 2036-6590
pISSN - 1972-2680
DOI - 10.3855/jidc.386
Subject(s) - salmonella , serotype , microbiology and biotechnology , biology , pulsed field gel electrophoresis , enterobacteriaceae , gene , bacteria , virology , genotype , genetics , escherichia coli
Three pediatric isolates of Salmonella serovar Typhimurium from Egypt were reported as CTX-M-14 ESBL producers. Two of the 3 isolates (68 and 111) were 85% related by PFGE;however both were distinct from isolate 94. All three isolates produced multiple clavulanate sensitive β-lactamase bands on IEF with pIs of 7.9 and 5.4 (all isolates), while pI 8.2 enzyme was produced by isolate 94 and pI 7.4 enzyme by isolate 111. The pI 7.9 enzyme was transferable by conjugation and conferred a CTX-M ESBL phenotype. PCR and sequencing identified the CTX-M gene as CTX-M-14. Genes for OXA-1 and SHV-12 were also identified by sequencing. To our knowledge this is the first report of CTX-M-producing Salmonella in Egypt.
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