Expression of ompR gene in the acid adaptation and thermal resistance of Salmonella Enteritidis SE86
Author(s) -
Ana Carolina Ritter,
Donatella Bacciu,
Lucélia Santi,
Salvatore Rubino,
Sergio Uzzau,
Eduardo César Tondo
Publication year - 2014
Publication title -
the journal of infection in developing countries
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 49
eISSN - 2036-6590
pISSN - 1972-2680
DOI - 10.3855/jidc.3584
Subject(s) - salmonella enteritidis , mutant , strain (injury) , microbiology and biotechnology , biology , chemistry , gene , salmonella , biochemistry , bacteria , genetics , anatomy
The objective of this study was to evaluate the involvement of the ompR gene in the acid adaptation and thermal resistance of S. Enteritidis SE86, responsible agent of more than 95 % of investigated food-borne diseases, throughout the last decade in Southern Brazil. In this study, we constructed a mutant strain of S. Enteritidis SE86 (ΔompR) that was attenuated by a knockout technique. The OmpR protein expression was determined in a tagged (3XFLAG) strain of S. Enteritidis SE86.
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