A new method for the capture of surface proteins in Plasmodium falciparum parasitized erythrocyte | Zendy

Emanuela Ferru | Zendy; Anntonella Pantaleo | Zendy; Francesco Turrini | Zendy

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A new method for the capture of surface proteins in Plasmodium falciparum parasitized erythrocyte

Author(s) -

Emanuela Ferru,

Anntonella Pantaleo,

Francesco Turrini

Publication year - 2012

Publication title -

the journal of infection in developing countries

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.322

H-Index - 49

eISSN - 2036-6590

pISSN - 1972-2680

DOI - 10.3855/jidc.2386

Subject(s) - biotinylation , avidin , biotin , plasmodium falciparum , streptavidin , membrane protein , biochemistry , chemistry , membrane , antigen , chromatography , biology , immunology , malaria

We propose a new method for the selective labeling, isolation and electrophoretic analysis of the Plasmodium falciparum protein exposed on the erythrocyte cell surface. Historically, membrane surface proteins have been isolated using a surface biotinylation followed by capture of biotin-conjugated protein via an avidin/streptavidin-coated solid support. The major drawback of the standard methods has been the labeling of internal proteins due to fast internalization of biotin.

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