Open AccessComparison of PCR with standard culture of fine needle aspiration samples in the diagnosis of tuberculosis lymphadenitis
Author(s) -
Yohannes Derese,
Elena Hailu,
Tekalign Assefa,
Yonas Bekele,
Adane Mihret,
Abraham Aseffa,
Jemal Hussien,
Ibrahim Ali,
Markos Abebe
Publication year - 2011
Publication title -
the journal of infection in developing countries
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 49
eISSN - 2036-6590
pISSN - 1972-2680
DOI - 10.3855/jidc.2050
Subject(s) - tuberculosis , gold standard (test) , medicine , cytology , bacilli , stain , fine needle aspiration , pathology , tuberculous lymphadenitis , mycobacterium tuberculosis , polymerase chain reaction , microbiological culture , staining , ziehl–neelsen stain , gram staining , biopsy , sputum , microbiology and biotechnology , biology , acid fast , bacteria , antibiotics , biochemistry , gene , genetics
Lymphadenopathy is the commonest form of extrapulmonary tuberculosis (TB) Clinical diagnosis of TB in lymph nodes requires aspiration of the material and isolation of mycobacteria. Bacterial culture is the gold standard for detection of tubercle bacilli, but it is time-consuming and requires specialized safety procedures and a BSL3 laboratory. However, PCR is a rapid method which requires small volumes of samples and can also be performed on killed bacilli to ensure safety. This project was designed to compare direct fine needle aspirate (FNA) PCR with culture in the diagnosis of tuberculosis lymphadenitis.
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