High-Resolution Melting and Real-Time Pcr for Quantification and Detection of Drug-Resistant HBV Mutants in a Single Amplicon | Zendy

ChiaChieh Hsiao | Zendy; Juliana Chang | Zendy; Jeng-You Wu | Zendy; Wan-Hsin Liu | Zendy; Shih-Yun Han | Zendy; PeiJer Chen | Zendy; Shiou-Hwei Yeh | Zendy

Open Access

High-Resolution Melting and Real-Time Pcr for Quantification and Detection of Drug-Resistant HBV Mutants in a Single Amplicon

Author(s) -

ChiaChieh Hsiao,

Juliana Chang,

Jeng-You Wu,

Wan-Hsin Liu,

Shih-Yun Han,

PeiJer Chen,

Shiou-Hwei Yeh

Publication year - 2011

Publication title -

antiviral therapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.747

H-Index - 87

eISSN - 2040-2058

pISSN - 1359-6535

DOI - 10.3851/imp2022

Subject(s) - entecavir , lamivudine , adefovir , high resolution melt , amplicon , hepatitis b virus , virology , mutant , population , viral load , biology , melting curve analysis , real time polymerase chain reaction , hepatitis b , medicine , virus , polymerase chain reaction , gene , genetics , environmental health

Antiviral therapy by nucleoside/nucleotide analogues (NAs) effectively reduces HBV replication in chronic hepatitis B (CHB) patients. Because long-term NA treatments will eventually select for drug-resistant mutants, early detection of mutants and frequent monitoring of viral loads is crucial for successful NA therapy. Because no efficient test for one-tube quantification and qualification of various HBV-resistant mutants exists, we propose to use high-resolution melting (HRM) analysis in combination with real-time PCR to achieve this unmet need.

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