Open AccessAnti-prM Antibody as an Autoantibody in Dengue Virus Infection
Author(s) -
Kao Jean Huang,
Yu Tien Cheng,
Yee Shin Lin,
Jyh Hsiung Huang,
Hsiao Sheng Liu,
Trai Ming Yeh,
Ching Chuan Liu,
Huan Lei
Publication year - 2008
Publication title -
american journal of infectious diseases
Language(s) - English
Resource type - Journals
eISSN - 1558-6340
pISSN - 1553-6203
DOI - 10.3844/ajidsp.2008.60.68
Subject(s) - dengue fever , autoantibody , dengue virus , antibody , virology , medicine , antibody dependent enhancement , immunology
We have reported that anti-prM antibody is an enhancing antibody that enhances DV infection of non-Fc receptor bearing cells by dual specificity. We identified the epitope recognized by this anti-prM antibody, M3, which is located at the a.a.53-67of the prM protein nearby the prM/M cleavage junction and these anti-M3 antibodies could be detected in dengue patients sera. Surprisingly, this anti-prM antibody not only recognizes the prM protein of dengue virions, but also cross-reacts with epithelial cells, endothelial cells as well as T cells. The binding of anti-prM antibody to endothelial cells was dose-dependent and could be blocked by M3 peptides. Human M3-specific antibodies from dengue patient sera were demonstrated to be able to bind to endothelial cells and mediate ADE infection on K562 cells. Furthermore, the anti-prM antibody will mediate the antibody -dependent cell phagocytosis, leading to the similar severe form of DHF/DSS. In conclusion, anti-prM antibody plays two roles in the pathogenesis of dengue virus infection: to be an enhancing antibody and an autoantibody as well