Open AccessEffect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum
Author(s) -
Mohd Sahaid Kalil,
Hisham Alshiyab,
Wan Mohtar Wan Yusoff
Publication year - 2008
Publication title -
american journal of biochemistry and biotechnology/american journal of biochemistry and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.161
H-Index - 31
eISSN - 1553-3468
pISSN - 1558-6332
DOI - 10.3844/ajbbsp.2008.393.401
Subject(s) - nitrogen , clostridium acetobutylicum , chemistry , carbon fibers , hydrogen , production (economics) , environmental science , biochemistry , mathematics , organic chemistry , economics , butanol , composite number , algorithm , ethanol , macroeconomics
Problem statement: One of the main factors influenced the bacterial
productivity and total yield of hydrogen is the nitrogen source and its concentration.
Approach: Using different nitrogen source with different concentration
on bacterial productivity of hydrogen showed to affect on both bacterial productivity
of hydrogen and biomass concentration. Results: Yeast extract
as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen
source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with
biomass concentration of 1.1 g L-1, hydrogen yield per biomass YP/X of 280 mL
g-1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in
gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S
from 308-350 mL g?1 glucose utilized with biomass concentration of 1.22 gL-1,
YP/X of 287 mL g-1 L-1, YX/S of 0.244 and YH2/S of 0.03125. Conclusion:
Nitrogen source with proper C:N ratio enhanced the hydrogen production.