Open AccessNoninvasive Measurement of Fecal Progesterone Concentration in Toy Poodles by Time Resolved Fluoroimmunoassay (TR-FIA)
Author(s) -
Satoshi Sugimura,
Kaori Narita,
Hideaki Yamashiro,
Atsushi Sugawara,
Katsuhiko Nishimori,
Tsutomu Konno,
Midori Yoshida,
Eimei Sato
Publication year - 2008
Publication title -
american journal of animal and veterinary sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.174
H-Index - 15
eISSN - 1557-4563
pISSN - 1557-4555
DOI - 10.3844/ajavsp.2008.43.46
Subject(s) - feces , chromatography , chemistry , biology , microbiology and biotechnology
Progesterone is an important reproductive hormone and measurement of its level by repeated blood samplings is beneficial to monitoring of estrus cycle. However, since toy poodles have a small body size and thin-walled blood vessels, repeated blood samplings cause stress and affect their preparation for mating or artificial insemination (AI). Therefore, a noninvasive method for monitoring progesterone concentration should be developed. Here, we show that time-resolved fluoroimmunoassay (TF-RIA) is a useful noninvasive method for determining the progesterone concentration in serum and fecal samples obtained from toy poodles. Present results demonstrate that progesterone concentrations in the fecal correlated with the serum collected in same time and the sequential changes in progesterone concentrations in the feces are paralleled in the serum. Therefore, this technique may be suitable for monitoring the estrus cycle in toy poodles