Detection of Glanders in horses of eight Iraqi provinces by ELISA | Zendy

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Detection of Glanders in horses of eight Iraqi provinces by ELISA

Author(s) -

Zaid Salah Hussein

Publication year - 2018

Publication title -

al-anbar journal of veterinary sciences

Language(s) - English

Resource type - Journals

eISSN - 2707-0603

pISSN - 1999-6527

DOI - 10.37940/ajvs.2018.11.1.4

Subject(s) - serology , veterinary medicine , medicine , statistical analysis , antibody , immunology , statistics , mathematics

The present study was carried out to determine the prevalence of Glanders in horses of Iraq. For this reason, a serological surveillance was conducted during the period extended between November 2015 and March 2016. Four hundred and four samples of serum were collected from horses lived in eight different Iraqi provinces that were Al-Muthanna, Baghdad, Al-Najaf, Babylon, Al-Qadsyia, Diyala, Karbala and Wasit. Sera transported to the lab of clinical pathology/ department of internal and preventive veterinary medicine at Baghdad University, where they were analyzed by cELISA technique to determine the seropositivity against Burkholderia mallei. The results of sera analysis showed that 2.97 % (12 horses) were positive toward the applied test. The positive cases were recorded in Baghdad and Muthanna governorate (11 and 1, respectively). Statistical analysis revealed no significant variations (P<0.05) in the seropositivity against Glanders between different genders or ages. Keywords: Glanders, Burkholderia mallei, Horse, ELISA, Epidemiology, Iraq e-mail: zaid.salah.hussein@gmail.com  ضرم نع يرحتلا يف لويخلا يف ماعرلا ةيقا رع تاظفاحم نامث ا زيللاا مادختساب نيسح حلاص ديز دادغب ةعماج /يرطيبلا بطلا ةيمك ةصلاخلا ده ت سد د ددكس ددل در ن عددكجد س رد نددكلرد ةد ععددلسرد اسددة سعدا ضد ددة عدد س ةر عد د س رد ا دده ة ددية مدد ة ءد ة ة ةةرد ةس فرد لال ةضعثرد كسا 2015 سد آ ىرد 2016 عهرلال ت ة 404 ية عضكل عك ةد ة د دن ة نكل ة د غ عدية د ا ده ديض عد د دنن ءلاغسدون ىرعدك ن عكد عيردن دغعغن بد ضردن د د غن ىدضثةرد ةهن عكجد سل تعظ عحة عكضعةث د د غ عد ةع ةد اسد كغرد ود رد عدكور طغعد رد اسد كغرد ةتعدجنردن ةض عدغرد ود رد لسد ةد تعديكلا رد سدغ لة ىدرد عده ة فر عدضه تدتلد ثدكح د دكر د دح ةد عض رد ( cELISA عدةنثس ر نديةرد عدكغع كج دل اسدح ر ) ددرد Burkholderia mallei . د جتع ضرد تسهظأ 2.07 ( نكلرد عيةد ة ٪ 12 ت د ت عدحرد ا ده طدكة حف ر عغ نة تضعو ) ية ىدضثةردن د د غ ة ظ عحة ة ( 1 ن 11 )ةردند رد ىد ل ع دل جتعد ض ر ةتعديح د دك ح رد سدهظد عدةو عدكنض ة نسد اأ ند ن (P<0.05) .ععلسرد اسةر نيةرد عكغع كلإ عغ ضرعغ سض رد ةعلن عف لةرد سعةل د كغ :عكحع فةرد تعة ورد ،ععلسرد اسة Burkholderia mallei ، ، نكلرد . د س رد ،د كر د Al-Anbar J. Vet. Sci., Vol.: 11 No. (1), 2018 ISSN: 1999-6527

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