Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus | Zendy

Huarong Li | Zendy; Wendy Sparks | Zendy; Bryony Bonning | Zendy

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Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

Author(s) -

Huarong Li,

Wendy Sparks,

Bryony Bonning

Publication year - 2008

Publication title -

journal of visualized experiments

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.596

H-Index - 91

ISSN - 1940-087X

DOI - 10.3791/889

Subject(s) - hemolymph , baculoviridae , biology , pest analysis , virology , larva , recombinant dna , ecology , botany , spodoptera , genetics , gene

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.

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