Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus | Zendy

Wendy Sparks | Zendy; Huarong Li | Zendy; Bryony C. Bonning | Zendy

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Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Author(s) -

Wendy Sparks,

Huarong Li,

Bryony C. Bonning

Publication year - 2008

Publication title -

journal of visualized experiments

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.596

H-Index - 91

ISSN - 1940-087X

DOI - 10.3791/888

Subject(s) - baculoviridae , biology , bioassay , pest analysis , virology , larva , computational biology , recombinant dna , genetics , gene , spodoptera , ecology , botany

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. This video shows how lepidopteran larvae can be infected with polyhedra by droplet feeding and diet plug-based bioassays. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents, including discussion of the pros and cons for use of baculoviruses as insecticides, and progress made in genetic enhancement of baculoviruses for improved insecticidal efficacy.

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