Live-imaging of Mitochondrial System in Cultured Astrocytes | Zendy

Jeanne Espourteille | Zendy; Valentin Zufferey | Zendy; Jean-Honoré Laurent | Zendy; Kevin Richetin | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Live-imaging of Mitochondrial System in Cultured Astrocytes

Author(s) -

Jeanne Espourteille,

Valentin Zufferey,

Jean-Honoré Laurent,

Kevin Richetin

Publication year - 2021

Publication title -

journal of visualized experiments

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.596

H-Index - 91

ISSN - 1940-087X

DOI - 10.3791/62957

Subject(s) - mitochondrion , mitochondrial biogenesis , astrocyte , live cell imaging , microbiology and biotechnology , biology , neuroscience , biogenesis , cell , biochemistry , central nervous system , gene

While much attention has been given to mitochondrial alterations at the neuronal level, recent evidence demonstrates that mitochondrial dynamics and function in astrocytes are implicated in cognition. This article describes the method for time-lapse imaging of astrocyte cultures equipped with a mitochondrial biosensor: MitoTimer. MitoTimer is a powerful and unique tool to assess mitochondrial dynamics, mobility, morphology, biogenesis, and redox state. Here, the different procedures for culture, image acquisitions, and subsequent mitochondrial analysis are presented.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research