Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ | Zendy

Jesús Espada | Zendy; Elisa Carrasco | Zendy; María I. Calvo-Sánchez | Zendy; Sandra Fernández-Martos | Zendy; Juan José Montoya | Zendy

Open Access

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Author(s) -

Jesús Espada,

Elisa Carrasco,

María I. Calvo-Sánchez,

Sandra Fernández-Martos,

Juan José Montoya

Publication year - 2020

Publication title -

journal of visualized experiments

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.596

H-Index - 91

ISSN - 1940-087X

DOI - 10.3791/60859

Subject(s) - protoporphyrin ix , reactive oxygen species , microbiology and biotechnology , hair follicle , photosensitizer , endogeny , regeneration (biology) , stimulation , stem cell , photodynamic therapy , chemistry , in vivo , biology , biophysics , biochemistry , neuroscience , photochemistry , genetics , organic chemistry

Here, we describe a protocol to induce switchable in vivo photogeneration of endogenous reactive oxygen species (ROS) in mouse skin. This transient production of ROS in situ efficiently activates cell proliferation in stem cell niches and stimulates tissue regeneration as strongly manifested through the acceleration of burn healing and hair follicle growth processes. The protocol is based on a regulatable photodynamic treatment that treats the tissue with precursors of the endogenous photosensitizer protoporphyrin IX and further irradiates the tissue with red light under tightly controlled physicochemical parameters. Overall, this protocol constitutes an interesting experimental tool to analyze ROS biology.

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