Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System | Zendy

Tatsuo Ito | Zendy; Keiki Ogino | Zendy; Kenjiro Nagaoka | Zendy; Kei Takemoto | Zendy; Riishiyama | Zendy; Yurika Shimizu | Zendy

Open Access

Detection of 3-Nitrotyrosine in Atmospheric Environments <em>via</em> a High-performance Liquid Chromatography-electrochemical Detector System

Author(s) -

Tatsuo Ito,

Keiki Ogino,

Kenjiro Nagaoka,

Kei Takemoto,

Riishiyama,

Yurika Shimizu

Publication year - 2019

Publication title -

journal of visualized experiments

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.596

H-Index - 91

ISSN - 1940-087X

DOI - 10.3791/58371

Subject(s) - chromatography , detection limit , high performance liquid chromatography , aerosol , chemistry , ozone , formic acid , particulates , nitrogen dioxide , organic chemistry

3-nitrotyrosine (3-NT) is generated from the tyrosine residue in atmospheric bio-aerosol proteins via a reaction with ozone (O3) and nitrogen dioxide (NO2). Stable 3-NT is a specific marker for oxidative damage and is reported to have a promotive effect to elicit allergies. In the present study, we report the development of a highly sensitive assay to quantify 3-NT in air sampler filters to collect < 2.5 µm of particulate matter (PM2.5) from urban environmental air, including bio-aerosol. In this method, a 6 mm-diameter round hole was cut from the filters of air samplers and mixed with a nonspecific protease cocktail in order to hydrolyze proteins. Protein samples digested to the amino acid level were tested for 3-NT using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD). The maximum 3-NT content was detected in a prefilter for PM of sizes from 4.5 to 7.3 μm, with a detection limit of 1.13 pg/m 3 .

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