Open AccessCreation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As<em> Escherichia Coli</em> or <em>Shigella flexneri</em>
Author(s) -
Nikki E. Freed
Publication year - 2017
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/56216-v
Subject(s) - transposable element , transposon mutagenesis , sleeping beauty transposon system , biology , shigella flexneri , plasmid , transposase , genetics , tn3 transposon , insertional mutagenesis , escherichia coli , genomic library , insertion , insertion sequence , genome , gene , mutant , base sequence
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