High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies | Zendy

Anup D. Sharma | Zendy; Pavel Brodskiy | Zendy; Emma M. Petersen | Zendy; Melih Dağdeviren | Zendy; EunAh Ye | Zendy; Surya K. Mallapragada | Zendy; Donald S. Sakaguchi | Zendy

Open Access

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Author(s) -

Anup D. Sharma,

Pavel Brodskiy,

Emma M. Petersen,

Melih Dağdeviren,

EunAh Ye,

Surya K. Mallapragada,

Donald S. Sakaguchi

Publication year - 2015

Publication title -

journal of visualized experiments

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.596

H-Index - 91

ISSN - 1940-087X

DOI - 10.3791/52242

Subject(s) - mesenchymal stem cell , neurotrophic factors , microbiology and biotechnology , neuroprotection , laminin , propidium iodide , stem cell , biology , glial cell line derived neurotrophic factor , cell , neural stem cell , brain derived neurotrophic factor , extracellular matrix , neuroscience , apoptosis , biochemistry , programmed cell death , receptor

doi:10.3791/52242 (2015). Mesenchymal stem cells (MSCs) derived from bone marrow are a powerful cellular resource and have been used in numerous studies as potential candidates to develop strategies for treating a variety of diseases. The purpose of this study was to develop and characterize MSCs as cellular vehicles engineered for delivery of therapeutic factors as part of a neuroprotective strategy for rescuing the damaged or diseased nervous system. In this study we used mouse MSCs that were genetically modified using lentiviral vectors, which encoded brain-derived neurotrophic factor (BDNF) or glial cell-derived neurotrophic factor (GDNF), together with green fluorescent protein (GFP). Before proceeding with in vivo transplant studies it was important to characterize the engineered cells to determine whether or not the genetic modification altered aspects of normal cell behavior. Different culture substrates were examined for their ability to support cell adhesion, proliferation, survival, and cell migration of the four subpopulations of engineered MSCs. High content screening (HCS) was conducted an

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