Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Author(s) -
T. Rama Reddy,
Emma J. Kelsall,
Léna M. S. Fevat,
Sarah E. Munson,
Shaun M. Cowley
Publication year - 2015
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/52155-v
Subject(s) - recombineering , gene targeting , biology , subcloning , bacterial artificial chromosome , homologous recombination , recombinase , genetics , computational biology , plasmid , gene , genome , recombination
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